Coding

Part:BBa_I715024

Designed by: Malcolm Campbell   Group: iGEM07_Davidson_Missouri_W   (2007-06-26)

Amino Half of CAT (aka 1Chlora)

This is the Amino half of Chloramphenicol Acetyltransferase which is 156 base pairs long. The gene was split at amino acid 52 because when the structure of the gene was observed, this point was far from the active site and therefore will more than likely not affect the overall function and structure of the gene. The eventual goal of the splitting is to put a HixC site at the end of this half of CAT and then ligate it to the other half (the carboxy end) and still have the gene work normally i.e. be resistant to Chloramphenicol.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None